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KMID : 0380020060210060422
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Korean Journal of Biotechnology and Bioengineering
2006 Volume.21 No. 6 p.422 ~ p.427
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Isolation and Characterization of Inhibition Helicobacter pylori from Culture Media of Fomitopsis pinicola
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Lee Jae-Kyoung
Choi Seong-Woo
Hwang Yu-Hyun
Park Hee-Kuk
Yoon Jeong-Weon
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Abstract
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The culture media from Fomitopsis pinicola were extracted by methanol and examined growth inhibition against Helicobacter pylori. The culture media from 8 days fermentation of F. pinicola showed maximum inhibition activity on H. pylori in 0.25 mg as MIC value. The highest activity against H. pylori by MHCS agar diffusion medium by Fp-P1 in 22.7 mm ID among 3 peaks from methanol fraction of 8 days culture media of Fomitopsis pinicola which purified by ion-exchange chromatography. The Fp-P1 from DEAE-Sephadex A-25 have been analysed by TLC as Fp-T1, Fp-T2 and Fp-T3 by ninhydrin staining. Fp-T3 (Rf value : 0.67) was higher activity against H. pylori in 14.4 mm ID. Fp-T3 was identified as single band by HPLC and TLC. It was assumed to aminosugar by BioLC analysis and TLC staining.
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KEYWORD
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Helicobactor pylori, Fomitopsis pinicola, antibiotics
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